General Session Information:
For 24-hour sessions, sessions will begin by 9:30AM on your scheduled day of imaging unless otherwise noted by staff. Weekend session grids are loaded on Friday evenings. During a new user training session, select a final grid by 2:00pm so staff have time to go through all the steps with you. 24-hour sessions and Rapid access sessions are independent and/or training sessions. Staff-assisted-screening sessions are fully operated by staff on the behalf of new users. For short screening sessions, please wait to hear from us for your official start time as they can be dependent on the previous user, or email cryoEM@crystal.harvard.edu .
Please review the appropriate protocols prior to your microscope session. Click the links below to view the protocols:
Single Particle Analysis (SPA):
>>> SerialEM User Guide and Troubleshooting Guide (Krios1 and Talos)<<<
>>> EPU User Guide (Krios2) <<<
>>> KRIOS-ONLY: Manually Centering Energy Filter (ZLP) <<<
Cryo-Electron Tomography (CryoET):
>>> Tomography Guide and Troubleshooting with SerialEM <<<
Dose Calculator (Choose SPA or CryoET option):
>>> Dose Calculator <<<
Common Magnifications on Krios1:
| Magnification (kx) | Pixel Size (Å/pixel) |
| 26 | ~3.34 |
| 33 | ~2.62 |
| 42 | ~2.14 |
| 53 | ~1.7 |
| 64 | 1.33 |
| 81 | 1.06 |
| 105 | 0.83 |
| 130 | 0.65 |
Common Magnifications on Krios2:
| Magnification | Krios 2 |
| 105kx | 1.19 |
| 130kx | 0.94 |
| 165kx | 0.73 |
Common Magnifications on Talos:
| Magnification | Talos Arctica |
| 36 kX | 1.10 Å/pix |