The Harvard Center for Cryo-Electron Microscopy (HC2EM) is a joint effort by Harvard Medical School, Dana-Farber Cancer Institute, Boston Children’s Hospital, and Massachusetts General Hospital to provide state-of-the-art cryo-EM instrumentation and expertise for the Harvard structural biology community. This user facility offers consultation and training by staff in specimen preparation, microscope operation, image acquisition, and data analysis. Currently, two MarkIV Vitrobots for sample preparation are available for use and we are accepting new users for the Talos Arctica and Titan Krios microscopes. The facility houses two Titan Krios election microscopes and a Talos Arctica electron microscope, all equipped with Gatan K3 Summit direct electron detectors.

Additionally, the Molecular Electron Microscopy Suite (MEMS) at Harvard Medical School is a separate user resource currently available to qualified researchers affiliated with Quad-based labs at HMS. This separate resource offers training and supervision in negative-stain and cryo-transmission electron microscopy. Equipment includes four transmission electron microscopes, two cryo plungers, and sample preparation areas. The Tecnai F20 and Polara F30 TEMs are each equipped with Gatan K2 Summit direct electron detectors.

If you have questions about access, services, or training, please email us at cryoem@crystal.harvard.edu.

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A Better Look: The development of cryo-EM has revolutionized structural biology

September 12, 2018
"Throughout history, observations of structure, from Hooke’s cells to the beaks of Darwin’s finches, have provided insights necessary to understand how life works. This is particularly true in structural biology, a discipline focused on visualizing life at its most fundamental. Discoveries of the atomic structures of important proteins and biological molecules have been among the most celebrated in science and have generated more than a dozen Nobel Prizes, new fields of research, and multibillion-dollar companies."
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