The Harvard Cryo-Electron Microscopy Center for Structural Biology is a joint effort by Harvard Medical School, Dana-Farber Cancer Institute, Boston Children’s Hospital, and Massachusetts General Hospital to provide state-of-the-art cryo-EM instrumentation and expertise for the Harvard structural biology community. This user facility offers consultation and training by staff in specimen preparation, microscope operation, image acquisition, and data analysis. Currently, two MarkIV Vitrobots for sample preparation are available for use and we are accepting new users for the Talos Arctica and Titan Krios microscopes. The facility houses 2 Titan Krios election microscopes and 1 Talos Arctica electron microscope, all equipped with Gatan K3 Summit direct electron detectors.
Additionally, the Molecular Electron Microscopy Suite at Harvard Medical School is a separate user resource currently available to qualified researchers affiliated with Quad-based labs at HMS. This separate resource offers training and supervision in negative-stain and cryo-transmission electron microscopy. Equipment includes 4 transmission electron microscopes, 2 cryo plungers, and sample preparation areas. The Tecnai F20 and Polara F30 TEMs are each equipped with Gatan K2 Summit direct electron detectors.