To request time on the microscopes in the Harvard Cryo-EM Center for Structural Biology, you will need to submit a project proposal. Please use the templates below. Give your project a brief title and then describe your project, including details about your specimen, a brief background, and the biological relevance of your project. For specimen screening on the Talos Arctica, you need to provide negative stain images and/or 2D classes and a description of your image collection and processing. For data collection on either the Talos Arctica or Titan Krios, you need to provide cryo images and 2D classes with a description of your image collection and processing scheme. Please reference the flowchart below for navigating the project proposal system. To submit a project proposal for review by your institutional committee, use the following link:
Click here to submit your proposal: Project Proposal Submission Form
After your insitutional committee has reviewed the proposals, facility staff will assign approved microscope time in PPMS (https://ppms.us/hms-cryoem/). You may log into PPMS using your eCommons ID and view the calendars for the Talos Arctica and both Titan Krios microscopes. If you need a PPMS account, fill out the form on our form page (https://cryoem.hms.harvard.edu/cemc-forms). Once your session is scheduled, your facility staff point of contact will email you regarding details about your session. Your point of contact can also work with you to adjust scheduled microscope time, answer questions about your session or facility procedures, and will make sure necessary paperwork is complete.
|Proposal template - Talos Arctica sample screening||14 KB|
|Proposal template - Titan Krios or Talos Arctica data collection||14 KB|