To request time on the microscopes in the Harvard Cryo-EM Center for Structural Biology, you will need to submit a project proposal. Please use the templates at the bottom of the page. Click here for Examples
Give your project a brief title and then describe your project, including details about your specimen, a brief background, and the biological relevance of your project. For specimen screening on the Talos Arctica, you need to provide biochemical data demonstrating homogenity or negative stain/cryo-em images. For data collection on either the Talos Arctica or Titan Krios, you need to provide cryo images or 2D classes with a description of your image collection and processing scheme.
Please reference the flowchart below for navigating the project proposal system.
For cryo-electron tomography, you need to provide information about your data processing scheme and pre-screened images of your grids.
*** Click here to submit your Project Proposal ***
After your institutional committee has reviewed and approved the proposal, you'll receive an email with detailed information about access to the facility and microscopes. You will be able to see the calendars for the microscope(s) and your project ID will appear in your PPMS account. There you'll be able to book a session, track the number of sessions you have, and upload POs as necessary.
If you have any questions about the submission forms or templates, you can email cryoem@crystal.harvard.edu.
Templates:
 | Proposal template - Talos Arctica sample screening | 14 KB |
| Proposal template - Titan Krios or Talos Arctica data collection | 14 KB |
| CryoET proposal template - Talos Arctica or Titan Krios | 14 KB |