Date:
Tuesday, November 6, 2018, 12:00pm to 1:00pm
Location:
SGM 106 & via webinar, see below for details
SBGrid Lunch Break with Sjors Scheres
RELION-3: new tools for automated high-resolution cryo-EM structure determination
Sjors Scheres
Group Leader
MRC Laboratory of Molecular Biology, UK
Tuesday, Nov 6: 12:00pm EST
Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, de novo model generation and 3D-classification. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher-resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. We illustrate these developments with publicly available data sets: together with a Bayesian approach to beam-induced motion correction it leads to resolution improvements of 0.2-0.7 Å compared to previous RELION versions.
Join SBGrid in SGM 106 or via the web: connection details here
Host: Jason Key
Event is also posted on SBGrid Google Calendar (to join visit http://sbgrid.org/calendar)