User Info

Harvard Cryo-EM Center for Structural Biology—Updated March 20th

Q: What equipment is currently available for use?

A: Two Vitrobots are available in the sample prep room located in SGM 102A. With project proposal submission and review, the Talos Arctica is available for sample screening and data collection.  The Titan Krios microscopes are available on an invitation-only basis. We will update this as more equipment is available.

Q: How do I get access to the equipment?

A: Our equipment will be available to all members of the Harvard, Boston Children's Hospital, Dana-Farber Cancer Institute, and Massachusetts General Hospital communities.

To access the Vitrobots, you can request a PPMS account via our submission form (PPMS Account Creation Form).  Once your account is active, you can schedule your preferred time. Your session will need to be approved by a staff member and we will provide a brief training during use. You can access and view the calendars here: https://ppms.us/hms-cryoem/.

To access the facility microscopes for sample screening or data collection, you need to submit a project proposal (https://cryoem.hms.harvard.edu/proposal).  Each proposal will be reviewed by a committee at the user's home institution.  Approved projects will be allowed 4 sessions of 24 hours each.  After these 4 sessions, users will need additional approval to book further sessions for the same project.  For more details, please see our policies page.

Q: How much does it cost to use the equipment?

A:  The following fees for equipment use and training have been approved.  Consumables such as clipping components, autogrid boxes, and hard drives will be charged at cost.

Talos Arctica $984 per day
Titan Krios $1560 per day
Microscope Training $250 per 4-hr session
(in addition to microscope cost)



 

 

 

Q: How much microscope time does my institution get?

A:

     

EM Suite @ HMS:

Q: What equipment is currently available for use?

A:  The CM10 and T12 microscopes are available to qualified users at Harvard Medical School.  We have moved all scheduling calendars to PPMS and will begin charging a fee for access and training on March 17.  Access for training on the F20 is on a request-only basis.  We are no longer training new users on the Polara.  The radioactive work area is available for negative stain sample preparation for users trained to use the microscopes in the EM Suite.

Fees for use:

  T12 CM10
Internal Harvard $13 per hour $10 per hour
External Academic $47 per hour $36 per hour
Training $250 per session $250 per session

For further information, please contact Zongli Li.