@article {Jenni2022,
	title = {Visualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle},
	journal = {Nature Communications},
	volume = {13},
	number = {1},
	year = {2022},
	month = {Aug},
	pages = {4802},
	abstract = {Vesicular stomatitis virus (VSV) is a negative-strand RNA virus with a non-segmented genome, closely related to rabies virus. Both have characteristic bullet-like shapes. We report the structure of intact, infectious VSV particles determined by cryogenic electron microscopy. By compensating for polymorphism among viral particles with computational classification, we obtained a reconstruction of the shaft ({\textquoteleft}{\textquoteleft}trunk{\textquoteright}{\textquoteright}) at 3.5{\th}inspace}\AA resolution, with lower resolution for the rounded tip. The ribonucleoprotein (RNP), genomic RNA complexed with nucleoprotein (N), curls into a dome-like structure with about eight gradually expanding turns before transitioning into the regular helical trunk. Two layers of matrix (M) protein link the RNP with the membrane. Radial inter-layer subunit contacts are fixed within single RNA-N-M1-M2 modules, but flexible lateral and axial interactions allow assembly of polymorphic virions. Together with published structures of recombinant N in various states, our results suggest a mechanism for membrane-coupled self-assembly of VSV and its relatives.},
	issn = {2041-1723},
	doi = {10.1038/s41467-022-32223-1},
	url = {https://doi.org/10.1038/s41467-022-32223-1},
	author = {Jenni, Simon and Horwitz, Joshua A. and Bloyet, Louis-Marie and Whelan, Sean P. J. and Harrison, Stephen C.}
}
