@article {Liu2022,
	title = {Sub-3-{\r A} cryo-EM structure of RNA enabled by engineered homomeric self-assembly},
	journal = {Nature Methods},
	year = {2022},
	month = {May},
	abstract = {High-resolution structural studies are essential for understanding the folding and function of diverse RNAs. Herein, we present a nanoarchitectural engineering strategy for efficient structural determination of RNA-only structures using single-particle cryogenic electron microscopy (cryo-EM). This strategy{\textendash}-ROCK (RNA oligomerization-enabled cryo-EM via installing kissing loops){\textendash}-involves installing kissing-loop sequences onto the functionally nonessential stems of RNAs for homomeric self-assembly into closed rings with multiplied molecular weights and mitigated structural flexibility. ROCK enables cryo-EM reconstruction of the Tetrahymena group I intron at 2.98-\AA resolution overall (2.85{\th}inspace}\AA for the core), allowing de novo model building of the complete RNA, including the previously unknown peripheral domains. ROCK is further applied to two smaller RNAs{\textendash}-the Azoarcus group I intron and the FMN riboswitch, revealing the conformational change of the former and the bound ligand in the latter. ROCK holds promise to greatly facilitate the use of cryo-EM in RNA structural studies.},
	issn = {1548-7105},
	doi = {10.1038/s41592-022-01455-w},
	url = {https://doi.org/10.1038/s41592-022-01455-w},
	author = {Liu, Di and Fran{\c c}ois A. Th{\'e}lot and Piccirilli, Joseph A. and Maofu Liao and Peng Yin}
}
