@article {Gui2021,
	title = {Structures of radial spokes and associated complexes important for ciliary motility},
	journal = {Nature Structural \& Molecular Biology},
	volume = {28},
	number = {1},
	year = {2020},
	month = {Jan},
	pages = {29-37},
	abstract = {In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two complementary cryo-EM strategies to determine structures of the major mechanoregulators that bind ciliary doublet microtubules in Chlamydomonas reinhardtii. We determine structures of isolated radial spoke RS1 and the microtubule-bound RS1, RS2 and the nexin-dynein regulatory complex (N-DRC). From these structures, we identify and build atomic models for 30 proteins, including 23 radial-spoke subunits. We reveal how mechanoregulatory complexes dock to doublet microtubules with regular 96-nm periodicity and communicate with one another. Additionally, we observe a direct and dynamically coupled association between RS2 and the dynein motor inner dynein arm subform c (IDAc), providing a molecular basis for the control of motor activity by mechanical signals. These structures advance our understanding of the role of mechanoregulation in defining the ciliary waveform.},
	issn = {1545-9985},
	doi = {10.1038/s41594-020-00530-0},
	url = {https://doi.org/10.1038/s41594-020-00530-0},
	author = {Gui, Miao and Ma, Meisheng and Sze-Tu, Erica and Wang, Xiangli and Koh, Fujiet and Zhong, Ellen D. and Berger, Bonnie and Davis, Joseph H. and Dutcher, Susan K. and Zhang, Rui and Brown, Alan}
}
