#  Cryo-EM Protocols 

 



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  [### Talos and Krios Protocols

 ](https://cryoem.hms.harvard.edu/talos-and-krios-protocols)User Guides and Pixels Sizing

 

   ![scopes](/sites/g/files/omnuum8871/files/styles/hwp_16_9__480x270/public/cryo-em.hms.harvard.edu/files/screen_shot_2021-05-22_at_10.27.56_am.png?itok=bKiydMqS) 

 

 

 

  [### Aquilos Protocols

 ](/aquilos-protocols)Training and User Guide

 

   ![Aquilos](/sites/g/files/omnuum8871/files/styles/hwp_16_9__480x270/public/2025-05/Screenshot%202025-05-07%20at%204.07.25%E2%80%AFPM.png?itok=Y3yjJnfL) 

 

 

 

  [### Vitrobot Protocols

 ](/vitrobot-rules)Freezing Room Guide

 

   ![sampleprep](/sites/g/files/omnuum8871/files/styles/hwp_16_9__480x270/public/cryo-em.hms.harvard.edu/files/img_1813.jpg?itok=48oayAo5) 

 

 

 

  [### Dewar & Sample Shipping

 ](/shipping) 

   ![shipping dewar](/sites/g/files/omnuum8871/files/styles/hwp_16_9__480x270/public/2025-05/Screenshot%202025-05-07%20at%204.11.49%E2%80%AFPM.png?itok=17fCyqxQ) 

 

 

 

  [### On-The-Fly (OTF) Processing for SPA

 ](https://www.dropbox.com/scl/fi/4ie8e1zfqdeqvkdjk729r/OTF_guide_Aug2022.pdf?rlkey=qv85q0qkxj8e2z6558f1m6cdr&dl=0)Click the link above for instructions to start the SBGrid pre-processing pipeline for SPA data.



 

   ![OTF Guide](/sites/g/files/omnuum8871/files/styles/hwp_16_9__480x270/public/2025-06/Screenshot%202025-06-11%20at%2011.08.53%E2%80%AFAM.png?itok=kfE8D7uB) 

 

 

 

  [### On-The-Fly (OTF) Processing for Cryo-ET

 ](https://www.dropbox.com/scl/fi/3kgegy64t897i7eqoryyc/FINAL_FOR_WEBSITE_OTF_tomo_guide_Nov2024.pdf?rlkey=64kezyuzrycgkj9lb76xbhuaa&dl=0)Click the link above to see the instructions for the starting the tomography pre-processing guide.



 

   ![virus](/sites/g/files/omnuum8871/files/styles/hwp_16_9__480x270/public/2025-05/viral.png?itok=TMfJ8jC5)