# Molecular Electron Microscopy Suite (MEMS) Training ## expand\_more ### **To sign up for training: please** [Request a PPMS account](/requesting-ppms-account)**. Once you have one, go to** [PPMS for MEMS](https://ppms.us/harvard-hms/) **and "request a training" (see image below). Staff will email you through the PPMS comment portal to schedule your training. Please see the microscope training sequence outlined below.** ![MEMS training](/sites/g/files/omnuum8871/files/styles/hwp_1_1__720x720_scale/public/cryo-em.hms.harvard.edu/files/screenshot_by_dropbox_capture.png?itok=UA2K1SSk) **All users are expected to follow the training process below to gain access to the MEMS. The user is expected to prepare grids with their own sample for training purposes. Staff will assist with training for negative stain grid preparation upon request. Please request a consultation in [PPMS](https://ppms.us/harvard-hms/?MEMS) if you would like training for grid preparation and microscope use. You can request a New User Onboarding Consultation in our HMS [CryoEM PPMS](https://ppms.us/harvard-hms). Once you have logged into our HMS CryoEM PPMS, there is an option on your Home page called 'request a training' (see image below); fill out and submit the training form titled "New User Onboarding". After you submit the form, a staff member will contact you to schedule. Those with Harvard IDs will be given badge access to the Sample Prep lab. If you are unsure of [how to obtain a Harvard ID](https://www.campusservicecenter.harvard.edu/services/id-cards/how-obtain-id-card), please contact your institution's administration. If you have any questions about this, please send them to email .** ## **CM10 training** provides an introduction to electron microscope operation and screening of negative stain grids. - Session 1: 2 hour introductory training session with staff. - Session 2: 2 hour training with staff; this will be a hands on session in which staff are present and watching. - Session 3 and beyond: independent sessions (without incident) - *Images with FFTs must be shared with staff to move to the T12 training.* ## **T12 negative stain training** provides the user with details on electron microscope operation by covering microscope alignment and low dose setup. - Session 1: 2 hour Training session with staff (loading and unloading the microscope; image acquisition.) - Session 2: 2 hour Hands-on session in which staff are present and watching. (loading and unloading the microscope; image acquisition) - Session 3 and beyond: Assisted session(s) as necessary for independent use; image acquisition and data collection. - *Images with FFTs must be shared with staff AND user must be able to load/unload grids independently in order to be able to move to the T12 cryo-holder training.* ## **T12 cryo training** is for people who have already learned how to prepare cryo grids on either the Vitrobots or CP3. The user will learn how to use the Gatan 626 cryo holder and dry pumping station for screening cryo grids. - Session 1: 4 hour Training session with staff (loading and unloading the cryo-holder to microscope; serialEM image acquisition) - Session 2: 4 hour Hands on session in which staff are present and watching. (loading and unloading the microscope; serialEM image acquisition - Session 3 and beyond: Assisted session(s) as necessary for independent use; (serialEM image acquisition and data collection). - Cryo-2D classes can be used to submit Talos or Krios proposal in CryoEM center. See more information here: - ## **F20 training is by request only.** F20 training builds on the general T12 operation, providing information about more micorscope alignments, direct electron detector training, use of SerialEM, and higher ordered screening of cryo samples (2D averages, low resolution 3D volumes). - 1 day shared session covering cryo holder usage and microscope alignments - 1 day shared session covering SerialEM account set up and training ## **Vitrobot training** is for people who have a sample that is ready for vitrification for cryoEM imaging. - 2 hour training session learning how to use the vitrobot. - This is done through the CryoEM center, please see the following link: