Titan Krios Protocols

 

Sessions will meet at SGM B04 to use the microscope during their session.  Click on a link below to view the protocol:

 

>>> Opening SerialEM & Starting LMMs <<<

>>> Krios data collection SerialEM setup (SPA) <<<

>>> Expanded Multi Shot Protocol <<<

>>> What to do if automatic energy filter centering (ZLP) fails <<<<

>>> Weekend independent User Guide <<<

>>> Krios SPA Data Collection Parameters Sheet <<<

>>> Krios Tomography Data Collection Parameters Sheet <<<

>>> Dose Calculator <<<

Common Magnifications on Krios1 and Krios2:

Magnification Krios 1 Krios 2
64kx 1.33 Å/pix 1.36 Å/pix
81kx 1.06 Å/pix 1.06 Å/pix
105kx 0.83 Å/pix 0.825 Å/pix
130kx 0.65 Å/pix 0.66 Å/pix