We house a two ThermoFisher Scientific Titan Krios operated at 300 keV each equipped with a Gatan BioQuantum GIF/K3 direct electron detector and Volta phase plates, as well as one ThermoFisher Scientific Talos Arctica operating at 200 kV equipped with Volta phase plate and Gatan K3 direct electron detector. Both are operated using Serial EM using automated protocols. VIew protocols here: Arctica Protocols.
For sample grid preparation and vitrification, we house a sample prep lab suite outfitted with an Pelco Easiglow plasma discharge system and 2 Vitrobot Mark IV freezing instruments.
For training or access to any of our instrumentation please email cryoEM@hms.harvard.edu or request a PPMS account and request an initial consultation.