#  SBGrid Lunch Break with Sjors Scheres 

 



####  calendar\_today Date and Time 

 **November 6, 2018** 

 12:00PM - 01:00PM EST 

####  pin\_drop Location 

 **SGM 106 & via webinar, see below for details**  



 

 



 

 SBGrid Lunch Break with Sjors Scheres

 **RELION-3: new tools for automated high-resolution cryo-EM structure determination**  Sjors Scheres

 Group Leader

 MRC Laboratory of Molecular Biology, UK

 Tuesday, Nov 6: 12:00pm EST

 *Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, de novo model generation and 3D-classification. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher-resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. We illustrate these developments with publicly available data sets: together with a Bayesian approach to beam-induced motion correction it leads to resolution improvements of 0.2-0.7 Å compared to previous RELION versions.* Join SBGrid in SGM 106 or via the web: connection details [ here](https://urldefense.proofpoint.com/v2/url?u=https-3A__sbgrid.org_news_sbgrid-2Dwebinar-2Dseries&d=DwMFaQ&c=WO-RGvefibhHBZq3fL85hQ&r=vMthnWFzRJvqgh_U7DKpZbvJ81borFFU1G7bS2hgVdQ&m=6JqRT6_-SwMg9NUDw-ox469CKsWDAyatA-SC9EqNHyc&s=uyG5jc1_PN4ZgLQsHzrzSe9t4-uEEoef54OFINZyihQ&e=)

 Host: Jason Key

 Event is also posted on SBGrid Google Calendar (to join visit [ http://sbgrid.org/calendar](https://urldefense.proofpoint.com/v2/url?u=http-3A__sbgrid.org_calendar&d=DwMFaQ&c=WO-RGvefibhHBZq3fL85hQ&r=vMthnWFzRJvqgh_U7DKpZbvJ81borFFU1G7bS2hgVdQ&m=6JqRT6_-SwMg9NUDw-ox469CKsWDAyatA-SC9EqNHyc&s=UWFoin2hKfJ6FrVdMFd3OuQG_Mn66QNlMen7EoJSRHY&e=))



 

 



 

 

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